Regulatory
Part:BBa_K1033223:Design
Designed by: Stephanie Herman, Alona Nyberg Group: iGEM13_Uppsala (2013-08-23)
Promoter CP30
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was based on a consensus sequence found in Lactococcus lactis. It was synthesized by using primers containing a overhang with the standard prefix and suffix.
Source
This part is a synthetic promoter based on a promoter library from the article "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." by Peter Ruhdal Jensen and Karin Hammer.
References
P. Jensen, K Hammer Appl: The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters Environ Microbiol. 64.1 (1998) 82–87